Zika virus IgG/IgM Rapid Test

ZVIRI KUSHANDISA

Zika virus IgG/IgM Rapid Test is a quick chromatographic immunoassay for qualitative kuonekwa kweIgG neIgM masoja ekudzivirira chirwere cheZika muropa revanhu rose, serum, kana plasma serubatsiro mukuongororwa kwezvirwere zveZika zvepuraimari nesekondari.

NHUNGAMIDZO

Zika fever, inozivikanwawo seZika virus disease kana kungoti Zika, chirwere chinotapukira chinokonzerwa neZika virus.1 Zviitiko zvizhinji hazvina zviratidzo, asi kana zviripo zvinowanzova zvinyoro uye zvinogona kuita se dengue fever.1-4 Zviratidzo zvinogona kusanganisira fivha. , maziso matsvuku, marwadzo emajoini, musoro, uye maculopapular rash. Zviratidzo zvinowanzopedza mazuva asingasviki manomwe.2 Hazvina kukonzera rufu chero rupi zvarwo panguva yehutachiwana hwekutanga.4 Kutapurirwa kubva kuna amai kubva kumwana panguva yekuzvitakura kunogona kukonzera microcephaly uye humwe hurema hwehuropi mune vamwe vacheche.5-6 Zvirwere muvanhu vakuru zvakabatanidzwa. kune Guillain-Barre syndrome. Serology yekuona chaiyo IgM uye IgG masoja ekudzivirira chirwere kuZika virus inogona kushandiswa. IgM antibodies inogona kuonekwa mukati memazuva matatu ekutanga kwechirwere.7 Serological cross-reactions neine hukama hweflaviviruses dzakadai se dengue uye hutachiona hweWest Nile pamwe chete nemishonga kune flaviviruses inogoneka.

Zika virus IgG/IgM Rapid Test bvunzo inokurumidza inoshandisa musanganiswa weZika antigen coated colored particles kuona IgG neIgM Zika masoja ekudzivirira chirwere muropa revanhu rose, serum, kana plasma.

PROCEDURE

Bvumira mudziyo wekuyedza, sampuli, buffer, uye/kana zvidhiraivho kuti zvisvike tembiricha yemumba (15 30°C) isati yaedzwa.

1. Hunza homwe yacho kuchidziya chekamuri usati wavhura. Bvisa mudziyo wekuyedza kubva muhomwe yakavharwa woishandisa nekukasika.
2. Isa mudziyo wekuyedza panzvimbo yakachena uye ine mwero.

ForSerum kana Plasma Mienzaniso: 

Bata chinodonhedza pasi, dhirowa mufananidzokusvika kuZadza Line (ingangoita gumi uL), uye endesa specimen kune specimen tsime (S) yemuyedzo mudziyo, wobva wawedzera madonhwe maviri ebhafa (ingangoita 80 mL) uye wotanga chibatiso chenguva. Ona mufananidzo uri pasi apa. Dzivisa kuteya mabhuru emhepo mumuenzaniso mutsime (S).

ForRopa Rakazara (Venipuncture/Fingerstick) Mienzaniso:

Kushandisa chinodonhedza: Bata chinodonhedza pasi, dhirowa mufananidzo0.5-1 cm pamusoro peKuzadza Line, uye woendesa madonhwe maviri eropa rose (inenge 20 µL) kumuenzaniso mutsime (S) wemudziyo wekuyedza, wobva wawedzera madonhwe maviri ebhafa (inenge 80 uL) wotanga chibatiso chenguva. Ona mufananidzo uri pasi apa.

Kushandisa micropipette: Pipette uye bvisa 20 µL yeropa rakazara kumuenzaniso mutsime (S) wemushini wekuyedza, wobva wawedzera madonhwe maviri ebuffer (ingangoita 80 µL) uye tanga iyo timer.

3. Mirira kuti mitsetse ine mavara ionekwe. Verenga mhinduro pamaminetsi gumi. Usadudzire mhinduro mushure memaminitsi makumi maviri.

DUDZIRIRO YEMIZVO