Trichomonas vaginalis Antigen Rapid Test

Short Description:

Trichomonas vaginalis Antigen Rapid Test is a rapid chromatographic immunoassay for the qualitative detection of Trichomonas vaginalis antigen in female cervical swab and male urethral swab specimen. The test results are intended to aid in the diagnosis of Trichomonas vaginalis infection in people. 

Used ForTrichomonas vaginalis Antigen Rapid Test

Specimenfemale cervical swab or male urethral swab

  1. CertificationCE
  2. MOQ1000

livery time2-5 days after Get payment
packing20 tests kits/Packing box

  1. Shelf Life24 Months

10.mentT/T, Western Union, Paypal

  1. Assay Time: 10-15 Minutes

Product Detail

Product Tags


SUMMARY

Trichomoniasis vaginalis is found all over the world. It is estimated that 3 million women in the United States and 180 million in the world are infected every year. Foreign data show that the infection rate of trichomonas is related to the number of sexual contact, and the infection rate of adult virgins is zero. In China, the infection rate of trichomonas in married women was about 20% in the 1950s, and the incidence rate reduced significantly in the 1970s. In recent years, due to the influence of sexual liberation, the incidence of vaginal trichomoniasis has increased in some foreign countries or regions, with the period of sexual exuberance as the susceptible age.

PRINCIPLE

Trichomonas vaginalis Antigen Rapid Test is a sandwich solid phase immunochromatographic assay. To perform the test, an aliquot of diluted sample is added to the sample well of the test cassette. The sample flows through a pad containing antibodies against Trichomonas vaginalis coupled to red-colored colloidal gold. If the sample contains Trichomonas vaginalis antigens, the antigen will bind to the antibody coated on the colloidal gold particles to form antigen-antibody-gold complexes. These complexes move on the nitrocellulose membrane by capillary action toward the test line region on which Trichomonas vaginalis antibodies are immobilized separately. As the complexes reach the test line, they will bind to the antibody corresponding to the bacteria on the membrane to form of a line. A red control line will always appear in the result window to indicate that the test has been correctly performed and the test device functions properly. If virus is not present or lower than the detection limit of the test, only the control line will be visible. If the control line dose not developed, the test is invalid.

MATERIALS

Materials Provided

  • Individually packed test devices
  • Dropper tips
  • Buffer
  • Workstation
  • Disposable sampling swabs (Female Cervical)
  • Extraction Tubes
  • Package insert

Materials Required but Not provided

  • Sterile Male Urethral Swabs
  • Timer

TEST PROCEDURE

Allow the test, reagents, swab specimen, and/or controls to reach room temperature (15-30°C) prior to testing.

  1. Remove the test cassette from the foil pouch and use it within one hour. Best results will be obtained if the test is performed immediately after opening the foil pouch.
  2. Extract the Trichomonas vaginalis antigen according to the specimen type.
  • For Female Cervical or Male Urethral Swab Specimen:
  • Hold the buffer bottle vertically and add 10 drops of buffer (approx. 300μl) to the extraction tube. Then immediately insert the swab, compress the bottom of tube and rotate swab 15 times. Let stand for 2 minutes.
  • Press the swab against the side of tube and withdraw the swab while squeezing the tube. Keep as much liquid in the tube as possible. Fit the dropper tip on top of extraction tube.
  1. Place the test cassette on a clean and level surface. Add 4 full drops of the extracted solution (approx. 100μl) to the specimen well of the test cassette, then start the timer. Avoid trapping air bubbles in the specimen well.
  2. Wait for the colored line(s) to appear. Read the result at 10 minutes; do not interpret the result after 20 minutes.

Note: It is recommended to use the buffer within 6 months after opening the vial.

INTERPRETATION OF RESULTS

POSITIVE: Two colored bands appear on the membrane. One band appears in the control region (C) and another band appears in the test region (T).

NEGATIVE: Only one colored band appears in the control region (C). No apparent colored band appears in the test region (T).

INVALID: Control band fails to appear. Results from any test which has not produced a control band at the specified read time must be discarded. Please review the procedure and repeat with a new test. If the problem persists, discontinue using the kit immediately and contact your local distributor.

NOTE:

  1. The intensity of color in the test region (T) may vary depending on the concentration of analytes present in the specimen. Therefore, any shade of color in the test region should be considered positive. Note that this is a qualitative test only, and cannot determine the concentration of analytes in the specimen.

 

  1. Insufficient specimen volume, incorrect operating procedure or expired tests are the most likely reasons for control band failure.

Relative Sensitivity: 94.12% (95%CI: 87.64%97.81%)

Relative Specificity: 97.58% (95%CI: 94.45%99.21%)

Accuracy: 96.44% (95%CI: 93.72%98.21%)



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