Zika virus IgG/IgM Rapid Test

UKUSETYENZISWA OKUHLOSIWEYO

Intsholongwane ye-Zika i-IgG/IgM Rapid Test yi-chromatographic immunoassay ekhawulezayo yokubona umgangatho we-IgG kunye ne-IgM ye-antibodies kwi-Zika virus egazini lomntu wonke, i-serum, okanye i-plasma njengoncedo ekuxilongweni kwe-Zika yokuqala kunye neyesibini.

INTSHAYELELO

I-Zika fever, eyaziwa ngokuba yi-Zika virus disease okanye i-Zika ngokulula, sisifo esosulelayo esibangelwa yintsholongwane kaZika.1 Uninzi lweziganeko azinampawu, kodwa xa zikhoyo zihlala zincinci kwaye zinokufana ne-dengue fever.1-4 Iimpawu zingabandakanya umkhuhlane. , amehlo abomvu, intlungu edibeneyo, intloko, kunye ne-maculopapular rash. Iimpawu ngokuqhelekileyo zihlala ngaphantsi kweentsuku ezisixhenxe.2 Akuzange kubangele nakuphi na ukufa okuchazwe ngexesha lokusuleleka kokuqala.4 Ukudluliselwa komama kumntwana ngexesha lokukhulelwa kunokubangela ukuba i-microcephaly kunye nezinye iziphene zengqondo kwezinye iintsana.5-6 Usulelo kubantu abadala luye lwadibaniswa kwi-Guillain-Barre syndrome. I-Serology yokufunyaniswa kwee-antibodies ze-IgM kunye ne-IgG kwi-virus ye-Zika ingasetyenziswa. Ii-antibodies ze-IgM zinokubonwa kwiintsuku ze-3 zokuqala kokugula.7 Iimpendulo ze-serological cross-reactions kunye ne-flaviviruses ezinxulumene ngokusondeleyo ezifana ne-dengue kunye ne-West Nile virus kunye nezitofu zokugonya i-flaviviruses zinokwenzeka.

Intsholongwane yeZika i-IgG/IgM Rapid Test luvavanyo olukhawulezayo olusebenzisa indibaniselwano yeZika antigen coated particles colored ukuze kubonwe i-IgG kunye ne-IgM ye-Zika antibodies kwigazi lomntu lonke, i-serum, okanye i-plasma.

INKQUBO

Vumela isixhobo sovavanyo, umfuziselo, isithinteli, kunye/okanye nezilawuli ukufikelela kubushushu begumbi (15 30°C) phambi kovavanyo.

1. Zisa isingxobo kwiqondo lobushushu begumbi phambi kokuvula. Susa isixhobo sovavanyo kwisingxobo esivaliweyo kwaye usisebenzise ngokukhawuleza.
2. Beka isixhobo sovavanyo kwindawo ecocekileyo nekumgangatho.

KubaIisampuli zeSerum okanye zePlasma: 

Bamba i-dropper ngokuthe nkqo, zoba umbonisoukuya kwiGcwalisa umgca (malunga ne-10 uL), kwaye udlulisele umboniso kumfanekiso wequla (S) wesixhobo sovavanyo, emva koko wongeze amathontsi ama-2 esikhuseli (malunga ne-80 mL) kwaye uqalise isibali-xesha. Jonga umfanekiso ongezantsi. Kuphephe ukubambisa amaqamza omoya kumfanekiso wequla (S).

KubaIGazi Elipheleleyo (I-Venipuncture/Fingerstick) Iisampulu:

Ukusebenzisa idropha: Bamba idropha ngokuthe nkqo, zoba umfuziselo0.5-1 cm ngaphezu komgca wokuZalisa, kwaye udlulisele amathontsi ama-2 egazi elipheleleyo (malunga nama-20 µL) kwisampulu yequla (S) lesixhobo sovavanyo, emva koko wongeze amathontsi ama-2 e-buffer (malunga nama-80 uL) kwaye uqalise isibali-xesha. Jonga umfanekiso ongezantsi.

Ukusebenzisa i-micropipette: I-Pipette kwaye ukhuphe i-20 µL yegazi elipheleleyo kwisampulu yequla (S) yesixhobo sovavanyo, uze wongeze amathontsi ama-2 e-buffer (malunga nama-80 µL) kwaye uqalise isibali-xesha.

3. Lindela ukuba umgca onemibala ibonakale. Funda iziphumo ngemizuzu eyi-10. Ungatoliki iziphumo emva kwemizuzu engama-20.

UKUTOLIKWA KWEZIPHUMO